Ronald E. McNair Program


Gregg Anazia
 School: University of Mississippi 
Major: Biochemistry
Mentor: Dr. Susan Pedigo, Assistant Professor of Chemistry
Expected Graduation Date: May 2005
Honors & Activities: 
  • Sally McDonnell-Barksdale Honors College
  • UAB School of Medicine – Automatic Interview
  • MAS – 2nd Place Presenter’s Award
  • Excellence in MS Scholar
  • IMAGE Scholar (Highest GPA Award 2003 - 2004)
  • Ronald E. McNair Scholar
  • MFGN Research Grant Recipient
  • Mortar Board – senior honor society
  • ASB (Elections Commission, Minority Affairs Committee Chair)
  • Omicron Delta Kappa – leadership honor society
  • Intramural Basketball & Football 
  • WhereFair Committee
  • World Fest Planning Committee
  • Student Affiliates of the American Chemical Society (President, Director of Public Relations)
  • Habitat for Humanity (Executive Vice President, Director of Public Relations)
  • Student Advancement Foundation
  • Celebrity Golf Classic (Marketing & Advertising Director, Sponsorship Director)
  • IMAGE (Internal Publicist)
  • William Winter Institute for Racial Reconciliation
  • NOBCChE
  • NSBE (Reporter)
  • Student Alumni Council (Special Events Committee)
  • Alpha Phi Omega (Vice President, Sergeant at Arms)
  • Chancellor’s Honor Roll
Email: goanazia@olemiss.edu

 

ABSTRACT

Shape vs. Size in Size Exclusion Chromatography


Cadherin is a cell adhesion protein that is significant in tissue formation.  Crystallographic studies indicate that the extracellular domains of cadherin are prolate ellipsoid in shape.  The molecular weight of two cadherin constructs as determined experimentally using size exclusion chromatography (SEC) was approximately twice that of the computed value.  This is misleading given the tendency for cadherin to dimerize in vivo.  Alternatively, the SEC column was calibrated according to the Stokes radius of the standard proteins.  Using this revised standard curve, cadherin constructs appeared to have Stokes radii very similar to that determined computationally for these constructs using the program HYDROPRO.  Thus, calibration of SEC columns according to the Stokes radius yields more reliable determination of the size of proteins.